Monoclonal antibodies against methyl glucuronoxylan epitopes for understanding xylan structure and modification

Lignocellulosic biomass is an abundant and renewable source that could be utilized for production of many bioproducts. It mainly consists cellulose, hemicellulose, lignin, all which come together to form a recalcitrant wall impedes In order overcome the nature biomass, it important fully understand how cell components are synthesized assembled. Xylan one hemicellulosic contributes recalcitrance. has substitutions on backbone formed by orchestration enzymes. For example, glucuronoxylan methyltransferases responsible 4-O-methylation glucuronic acid (GlcA) side chains xylan. Mutant lines valuable sources structure importance Here, we investigated wild-type gxm1gxm2gxm3 triple mutant Arabidopsis employing set four xylan antibodies specifically recognize methyl epitopes prove their epitope. The walls were observed with specific monoclonal first time. Cell-wall-directed help further our understanding synthesis plant walls.